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Detection of Legionella spp. in cooling tower water by the polymerase chain reaction method. |
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| Authors: | M Koide A Saito N Kusano and F Higa |
| Abstract: | The presence of Legionella spp. in cooling tower water was investigated by using the polymerase chain reaction. Total Legionella spp. detection was performed with 20-mer 5S rRNA complementary DNA sequence primers, and specific Legionella pneumophila detection was performed with 20-mer and then 21-mer macrophage infectivity potentiator gene sequence primers. Of 27 cooling tower water samples, 25 were positive for Legionella spp., and 14 of these contained L. pneumophila. |
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