Biphasic Stimulation of Translational Activity Correlates with Induction of Translation Elongation Factor 1 Subunit [alpha] upon Wounding in Potato Tubers |
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| Authors: | Morelli J K Shewmaker C K Vayda M E |
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| Affiliation: | Department of Biochemistry, Microbiology and Molecular Biology, University of Maine, Orono, Maine 04469-5735 (J.K.M, M.E.V). |
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| Abstract: | Potato (Solanum tuberosum) tubers exhibit an increase in translational activity in response to mechanical wounding. The response is biphasic, with an initial stimulation apparent within the first 2 h after wounding and a second increase occurring 12 to 24 h after wounding. Increased activity is apparent by measurement of protein synthesis both in vivo and in vitro using a cell-free extract. Accumulation of the translational elongation factor 1 subunit alpha] (EF-1alpha]) parallels translational activity. Changes in the steady-state level of EF-1alpha] mRNA, and expression of a chimeric EF-1alpha] promoter/beta]-glucuronidase construct in transgenic potato tubers, indicate that the gene encoding EF-1alpha] is transcribed during both periods of translational stimulation. These results indicate that stimulation of translational activity is coordinated with increased expression and accumulation of translation factors. |
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