Cell density and airspace patterning in the leaf can be manipulated to increase leaf photosynthetic capacity |
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| Authors: | Andrew Mathers Jen Sloan Marion Bauch Alice Mitchell Chandra Bellasio Adam Green Daniel Bouyer Arp Schnittger Craig Sturrock Colin P Osborne Stephen Rolfe Sacha Mooney Andrew J Fleming |
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| Affiliation: | 1. Division of Agriculture and Environmental Sciences, School of Biosciences, University of Nottingham, Loughborough, UK;2. Department of Animal and Plant Sciences, University of Sheffield, Sheffield, UK;3. Department of Physics and Astronomy, University of Sheffield, Sheffield, UK;4. Institut de Biologie Moleculaire des Plantes du CNRS, IBMP‐CNRS‐UPR2357, Strasbourg, France |
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| Abstract: | The pattern of cell division, growth and separation during leaf development determines the pattern and volume of airspace in a leaf. The resulting balance of cellular material and airspace is expected to significantly influence the primary function of the leaf, photosynthesis, and yet the manner and degree to which cell division patterns affect airspace networks and photosynthesis remains largely unexplored. In this paper we investigate the relationship of cell size and patterning, airspace and photosynthesis by promoting and repressing the expression of cell cycle genes in the leaf mesophyll. Using microCT imaging to quantify leaf cellular architecture and fluorescence/gas exchange analysis to measure leaf function, we show that increased cell density in the mesophyll of Arabidopsis can be used to increase leaf photosynthetic capacity. Our analysis suggests that this occurs both by increasing tissue density (decreasing the relative volume of airspace) and by altering the pattern of airspace distribution within the leaf. Our results indicate that cell division patterns influence the photosynthetic performance of a leaf, and that it is possible to engineer improved photosynthesis via this approach. |
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| Keywords: | leaf cell division photosynthesis
Arabidopsis thaliana
differentiation |
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