A Bacillus megaterium Plasmid System for the Production, Export, and One-Step Purification of Affinity-Tagged Heterologous Levansucrase from Growth Medium |
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| Authors: | Marco Malten Rebekka Biedendieck Martin Gamer Ann-Christin Drews Simon Stammen Klaus Buchholz Lubbert Dijkhuizen and Dieter Jahn |
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| Abstract: | A multiple vector system for the production and export of recombinant affinity-tagged proteins in Bacillus megaterium was developed. Up to 1 mg/liter of a His6-tagged or Strep-tagged Lactobacillus reuteri levansucrase was directed into the growth medium, using the B. megaterium esterase LipA signal peptide, and recovered by one-step affinity chromatography. |
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