Solubilization and characterization of substance P-binding sites from chick brain membranes. |
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| Authors: | H P Too and M R Hanley |
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| Affiliation: | Department of Biological Chemistry and Molecular Pharmacology, Harvard Medical School, Boston, MA 02115. |
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| Abstract: | Sites binding monoiodinated-Bolton-Hunter-reagent-labelled substance P were solubilized from 1-day-old-chick brain membrane by using non-ionic detergents (1% digitonin/1% n-octyl glucoside) and a high concentration of NaCl (0.5 M). The solubilized preparation retained the pharmacological properties of the high-affinity binding sites found in the native membrane. The high density of specific binding sites (approximately 2 pmol of binding sites/mg of protein) suggests that the chick brain membranes may be a useful source for the purification of the substance P-binding sites. |
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