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Assembly of LamB and OmpF in deep rough lipopolysaccharide mutants of Escherichia coli K-12. |
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| Authors: | M W Laird A W Kloser R Misra |
| Abstract: | Assembly of the OmpF and LamB proteins was kinetically retarded in deep rough lipopolysaccharide mutants of Escherichia coli K-12. OmpF assembly was affected at the step of conversion of metastable trimers to stable trimers, whereas LamB assembly was influenced both at the monomer-to-metastable trimer and metastable-to-stable trimer steps. These assembly defects were reversed in the presence of the sfaA1 and sfaB3 suppressor alleles, which were isolated by using ompF assembly mutants. |
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