Spectral evidence for a component involved in hydrogen metabolism of soybean nodule bacteroids |
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Authors: | Eisbrenner G Evans H J |
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Affiliation: | Laboratory for Nitrogen Fixation Research, Oregon State University, Corvallis, Oregon 97331. |
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Abstract: | A component with a difference spectrum similar to that of b-type cytochromes which becomes reduced upon the addition of H2 has been demonstrated in soybean nodule bacteroids. This electron carrier, referred to as component 559-H2, is present in hydrogenase-positive strains of Rhizobium japonicum but has not been detected in mutants that lack hydrogenase activity or in hydrogenase-negative wild-type strains. A positive correlation between concentrations of component 559-H2 and hydrogenase activities has been established. These results provide further evidence that component 559-H2 is involved in H2 metabolism in R. japonicum. |
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