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TiLIA: a software package for image analysis of firefly flash patterns |
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| Authors: | Junsuke Konno Yoko Hatta‐Ohashi Ryutaro Akiyoshi Anchana Thancharoen Somyot Silalom Watana Sakchoowong Vor Yiu Nobuyoshi Ohba Hirobumi Suzuki |
| Affiliation: | 1. Advanced Analysis Technology Department, Olympus Corporation, Hachioji, Tokyo, Japan;2. Department of Entomology, Faculty of Agriculture, Kasetsart University, Chatuchak, Bangkok, Thailand;3. Entomology Section, Queen Sirikit Botanic Garden, Maerim, Chiang Mai, Thailand;4. Department of National Parks, Wildlife and Plant Conservation, Forest and Plant Conservation Research Office, Chatuchak, Bangkok, Thailand;5. Hong Kong Entomological Society, Yuen Long, Hong Kong, China;6. The Ohba Firefly Institute, Yokosuka, Kanagawa, Japan |
| Abstract: | As flash signaling patterns of fireflies are species specific, signal‐pattern analysis is important for understanding this system of communication. Here, we present time‐lapse image analysis (TiLIA), a free open‐source software package for signal and flight pattern analyses of fireflies that uses video‐recorded image data. TiLIA enables flight path tracing of individual fireflies and provides frame‐by‐frame coordinates and light intensity data. As an example of TiLIA capabilities, we demonstrate flash pattern analysis of the fireflies Luciola cruciata and L. lateralis during courtship behavior. |
| Keywords: | Flash communication flight path tracing time‐lapse image analysis |
