| 海藻酸微囊在细胞冷冻保存中的研究现状及应用 |
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| 引用本文： | 刘代艳,萨初拉,吴应积.海藻酸微囊在细胞冷冻保存中的研究现状及应用[J].现代生物医学进展,2012,12(34):6785-6787. |
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| 作者姓名： | 刘代艳 萨初拉 吴应积 |
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| 作者单位： | 内蒙古大学哺乳动物生殖生物学与生物技术教育部重点实验室 内蒙古呼和浩特010021 |
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| 基金项目： | 内蒙古自治区自然科学基金项目(2009ZD05);教育部创新团队项目(IRT0833) |
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| 摘 要： | 细胞的冷冻保存是细胞生物学实验中重要的实验技术.长期以来,人们使用冷冻保存液重悬细胞后进行冷冻储存,但是近年来,众多研究者发现传统冷冻方案往往会导致细胞活率大幅下降和细胞功能方面受损,从而很难满足生物医学、组织再生工程、细胞移植技术等高新技术的要求.所以研究者提出利用三维海藻酸微囊包埋细胞后再进行冷冻保存,从而在保证较高细胞活率的同时维持细胞的原有功能,有效的提高细胞的冷冻保存效率.本文概述了海藻酸微囊在细胞冷冻保存过程中的研究现状,同时对其应用进行了展望,以期为后续研究工作提供参考.
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| 关 键 词： | 海藻酸微囊 冷冻保存 细胞活率 生物医学 |
The Current Research Situation and Applications of Alginate Microcapsules in Cells Cryopreservation |
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| LIU Dai-yan,SA Chu-la,WU Ying-ji.The Current Research Situation and Applications of Alginate Microcapsules in Cells Cryopreservation[J].Progress in Modern Biomedicine,2012,12(34):6785-6787. |
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| Authors: | LIU Dai-yan SA Chu-la WU Ying-ji |
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| Affiliation: | (Key Laboratory of Education Ministry of China for Mammalian Reproductive Biology and Biotechnology,University of Inner Mongolia,Hohhot,010021,China) |
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| Abstract: | Cells cryopreservation plays a critical role in the cell biological technology.Fora long time,people used cryopreservation liquid to suspension cells before cryopreservation,but in recent years,many researchers have found that the traditional frozen solutions often lead to substantial decline of cell viability and loss of important cellular functions,which is hard to meet the biomedical,tissue regeneratve project,cells transplantation and other high-technical requirements.Therefore,researchers have proposed to apply three-dimensional alginate microcapsules containing embedded cells before cryopreservation,which ensure higher cellular viability and no loss of main cellular functions,which improve the efficiency of cell cryopreservation.This article summarizes the researches and applications of alginate microcapsules in the process of cryopreservation,At the same time,it made application prospect,so as to provide reference for follow-up study. |
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| Keywords: | Alginate microcapsules Cryopreservation Cellular viability Cellular functions Biomedical |
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