Construction of a novel turn‐on–off fluorescence sensor used for highly selective detection of thiamine via its quenching effect on o‐phen‐Zn2+ complex |
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| Authors: | Hui Zhang Hongyun Chen Hongxi Li Shuang Pan Yalin Ran Xiaoli Hu |
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| Affiliation: | Key Laboratory of Luminescent and Real‐time Analytical Chemistry (Southwest University), Ministry of Education, College of Chemistry and Chemical Engineering, Southwest University, Chongqing, P. R. China |
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| Abstract: | In this work, a simple and selective fluorescence sensor approach called ‘turn‐on–off’ for the determination of thiamine (TM) has been developed. As known, the o‐phenanthroline (o‐phen) has inner fluorescence, though when reacted with zinc ions to form the o‐phen–Zn2+ complex the fluorescence intensity was enhanced effectively, while upon addition of TM into the o‐phen–Zn2+ complex solution, the intensity of the system was gently quenched, which was termed the ‘turn‐on–off’ probe. Notably, the method possessed highly selective, sensitive determination for TM with a detection limit of 0.25 μmol L?1 and the reduced fluorescence intensity was proportional to the concentration of TM in the range 0.84–80.0 μmol L?1. Besides, the proposed mechanism was also investigated through exploring the Fourier transform infrared (FT‐IR), nuclear magnetic resonance (NMR) spectroscopy. Furthermore, this manner was successfully applied into practical samples for TM detection with satisfactory results. |
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| Keywords: | detection fluorescence sensor o‐phen‐Zn2 + thiamine turn‐on– off |
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