Comparison of Thermosensitive Alleles of the Cdc25 Gene Involved in the Camp Metabolism of Saccharomyces Cerevisiae |
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| Authors: | A Petitjean F Hilger and K Tatchell |
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| Affiliation: | Faculté des Sciences Agronomiques, Laboratoire de Microbiologie, Gambloux, Belgium. |
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| Abstract: | The CDC25 gene from Saccharomyces cerevisiae is an essential component of the RAS-adenylate cyclase pathway. Genetic and biochemical evidence has led to the proposal that the gene product may act upstream of RAS, possibly as a guanine nucleotide exchange factor. We report here the cloning, sequencing and characterization of four mutations in the CDC25 gene. All four are missense mutations which reside within the carboxy-terminal quarter of the single open reading frame found within the gene. Three of the four are missense mutations in the same amino acid codon. A search of protein data bases reveals that the carboxy terminus of the putative CDC25 gene product is similar to that of LTE1, a gene required for growth at low temperature and SCD25, a suppressor of cdc25. Taken together these data indicate that the carboxy terminus of CDC25 plays a critical role in the function of the CDC25 gene product and that other proteins, such as LTE1 or SCD25, may have related activities. |
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