Nucleotide sequence of a neurovirulent variant of the type 2 oral poliovirus vaccine. |
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| Authors: | S R Pollard G Dunn N Cammack P D Minor J W Almond |
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| Affiliation: | Department of Microbiology, University of Reading, England. |
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| Abstract: | Infectious cDNAs of the Sabin type 2 poliovirus vaccine virus and a vaccine-derived neurovirulent type 2 strain (P2/117) have been cloned in Escherichia coli. Nucleotide sequence analysis revealed that P2/117 differs from the vaccine strain by just 23 point mutations. Three occur in the 5' noncoding region. The remainder result in a total of 5 coding changes located in VP1, VP4, 2B, and 3D. The likely role of these mutations in the evolution to neurovirulence is discussed. |
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