Genetic resources offer efficient tools for rice functional genomics research |
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| Authors: | Shuen‐Fang Lo Ien‐Chie Wen Yi‐Lun Liu Ku‐Ting Chen Mirng‐Jier Jiang Ming‐Kuang Lin Meng‐Yen Rao Lin‐Chih Yu Tuan‐Hua David Ho Su‐May Yu |
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| Affiliation: | 1. Institute of Molecular Biology, Academia Sinica, Taipei, Taiwan, ROC;2. Agricultural Biotechnology Center, National Chung Hsing University, Taichung, Taiwan, ROC;3. Plant Germplasm Division, Taiwan Agricultural Research Institute, Taichung, Taiwan, ROC;4. Institute of Plant and Microbial Biology, Academia Sinica, Taipei, Taiwan, ROC;5. Department of Life Sciences, National Chung Hsing University, Taichung, Taiwan, ROC |
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| Abstract: | Rice is an important crop and major model plant for monocot functional genomics studies. With the establishment of various genetic resources for rice genomics, the next challenge is to systematically assign functions to predicted genes in the rice genome. Compared with the robustness of genome sequencing and bioinformatics techniques, progress in understanding the function of rice genes has lagged, hampering the utilization of rice genes for cereal crop improvement. The use of transfer DNA (T‐DNA) insertional mutagenesis offers the advantage of uniform distribution throughout the rice genome, but preferentially in gene‐rich regions, resulting in direct gene knockout or activation of genes within 20–30 kb up‐ and downstream of the T‐DNA insertion site and high gene tagging efficiency. Here, we summarize the recent progress in functional genomics using the T‐DNA‐tagged rice mutant population. We also discuss important features of T‐DNA activation‐ and knockout‐tagging and promoter‐trapping of the rice genome in relation to mutant and candidate gene characterizations and how to more efficiently utilize rice mutant populations and datasets for high‐throughput functional genomics and phenomics studies by forward and reverse genetics approaches. These studies may facilitate the translation of rice functional genomics research to improvements of rice and other cereal crops. |
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| Keywords: | functional genomics gene activation gene knockout promoter trapping rice T‐DNA |
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