Primary structure analysis of an integral membrane glycoprotein of the nuclear pore |
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| Authors: | R W Wozniak E Bartnik G Blobel |
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| Affiliation: | Laboratory of Cell Biology, Howard Hughes Medical Institute, Rockefeller University, New York 10021. |
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| Abstract: | The complete primary structure of an integral membrane glycoprotein of the nuclear pore was deduced from the cDNA sequence. The cDNA encodes a polypeptide of 204,205 D containing a 25-residue-long signal sequence, two hydrophobic segments that could function as transmembrane segments, and 13 potential N-linked oligosaccharide addition sites. Endoglycosidase H reduces the molecular mass by approximately 9 kD suggesting that not all of these 13 sites are used. We discuss possible models for the topology of this protein in the pore membrane as well as a possible role in the formation of pores and pore complexes. |
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