Amplification and expression of heterologous ornithine decarboxylase in Chinese hamster cells. |
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| Authors: | T R Chiang and L McConlogue |
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| Affiliation: | Cetus Corporation, Emeryville, California 94608. |
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| Abstract: | ![]()
We have developed an amplifiable mammalian expression vector based on the enzyme ornithine decarboxylase (ODC). We show greater than 700-fold amplification of this vector in ODC-deficient Chinese hamster ovary cells. A passive coamplified marker, dihydrofolate reductase (dhfr), was amplified and overexpressed 1,000-fold. This ODC vector was a dominant marker in a variety of cell types and displayed at least 300-fold amplification in wild-type Chinese hamster ovary cells. |
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