| 剪切应力环境下的内皮祖细胞对肝星状细胞增殖和生物功能的影响 |
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| 引用本文： | 陈信信,张晓芸,丁玉真,李鑫,官秀梅,李宏,成敏,崔晓栋.剪切应力环境下的内皮祖细胞对肝星状细胞增殖和生物功能的影响[J].中国应用生理学杂志,2018,34(5):404-407. |
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| 作者姓名： | 陈信信 张晓芸 丁玉真 李鑫 官秀梅 李宏 成敏 崔晓栋 |
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| 作者单位： | 1. 潍坊医学院 生物科学与技术学院, 山东 潍坊 261053;
2. 潍坊医学院 临床医学院, 山东 潍坊 261053 |
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| 基金项目： | 国家自然科学基金（31570941，81700406，31270993）；山东省自然科学基金（ZR2014JL018，ZR2016CM20）；山东省高等学校科技计划项目（J15LK08，J14LK59，J14LK12）；国家大学生创新计划项目（201510438013）；潍坊医学院大学生科技创新基金（KX2016021，KX2015013）；山东省中医药科技发展计划项目（2015-239）；山东省医药卫生科技发展计划项目资助（2016WS0667） |
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| 摘 要： | 目的：研究流体剪切应力条件下的内皮祖细胞（EPCs）对肝星状细胞（HSCs）增殖、粘附、迁移、凋亡等生物学功能以及成纤维化因子α-平滑肌肌动蛋白（α-SMA）、胶原I （Col-I）、胶原III （Col-III）表达的影响。方法：将HSCs与EPCs分别接种于共培养小室的上层和下层，共培养24 h后，给EPCs细胞施加12 dyne/cm2剪切应力，持续24 h。消化细胞，采用CCK-8法检测HSCs的增殖；流式细胞术检测HSCs的凋亡率；细胞贴壁法检测HSCs的粘附功能；Boyden小室检测HSCs的迁移；荧光定量PCR法及Western blot分别检测HSCs的α-SMA、Col-I、Col-III mRNA和蛋白质的表达情况。结果：在剪切应力条件下，EPCs生态小境能明显抑制HSCs的增殖、粘附和迁移能力，促进HSCs凋亡，下调HSCs中Col-I、Col-III mRNA和蛋白质的表达。结论：在剪切应力条件下，EPCs生态小境对HSCs纤维化的发展具有一定抑制作用。
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| 关 键 词： | 内皮祖细胞 肝星状细胞 剪切应力 共培养 生态小境 |
| 收稿时间： | 2017-08-28 |
Effects of endothelial progenitor cells on proliferation and biological function of hepatic stellate cells under shear stress |
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| CHEN Xin-xin,ZHANG Xiao-yun,DING Yu-zhen,LI Xin,GUAN Xiu-mei,LI Hong,CHENG Min,CUI Xiao-dong.Effects of endothelial progenitor cells on proliferation and biological function of hepatic stellate cells under shear stress[J].Chinese Journal of Applied Physiology,2018,34(5):404-407. |
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| Authors: | CHEN Xin-xin ZHANG Xiao-yun DING Yu-zhen LI Xin GUAN Xiu-mei LI Hong CHENG Min CUI Xiao-dong |
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| Affiliation: | 1. College of Biological Science and Technology, Weifang Medical University, Weifang 261053, China;
2. Clinical Medical College, Weifang Medical University, Weifang 261053, China |
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| Abstract: | Objective: To investigate the effects of endothelial progenitor cells (EPCs) under shear stress on the biological function such as proliferation, adhesion, migration, apoptosis and expression of α-smooth muscle actin (α-SMA), collagen-I and collagen-Ⅲ of hepatic stellate cells (HSCs). Methods: HSCs and EPCs were inoculated into the upper and lower layers of the co-culture chamber respectively and co-incubated for 24 hours. Then, 12 dyne/cm2 shear stress was applied to EPCs cells for another 24 hours. After that, proliferation, adhesion, migration and apoptosis of HSCs were detected by cell counting kit-8 (CCK-8) kit, cell adherent assay, Boyden cell migration assay and flow cytometry respectively. Fluorescence quantitative PCR and Western blot were used to detect the mRNA and protein expression of alpha -SMA, collagen I and collagen-Ⅲ in HSCs. Results: Under shear stress, EPCs ecological niche could obviously inhibit the proliferation, adhesion and migration of HSCs, promote the apoptosis of HSCs, and down-regulate the mRNA and protein expression of collagen-I, collagen-Ⅲ in HSC cells. Conclusion: Under shear stress, EPCs ecological niche could inhibit the fibrosis development of HSCs to a certain extent. |
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| Keywords: | endothelial progenitor cells hepatic stellate cells shear stress co-culture ecological niche |
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