| HLA-G阳性的胎盘间充质干细胞体外诱导Treg的实验研究 |
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| 引用本文： | 崔桂玉,白剑,苗兰英,林大勇,刘鸿,李亚丽,刘喜成.HLA-G阳性的胎盘间充质干细胞体外诱导Treg的实验研究[J].中国应用生理学杂志,2018,34(5):396-400. |
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| 作者姓名： | 崔桂玉 白剑 苗兰英 林大勇 刘鸿 李亚丽 刘喜成 |
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| 作者单位： | 1. 内蒙古民族大学医学院, 通辽 028000;
2. 辽宁中医药大学, 沈阳 110847;
3. 深圳市人民医院麻醉科, 广东 深圳 518020;
4. 深圳市麻醉医学工程研究中心, 广东 深圳 518020 |
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| 基金项目： | 国家自然科学基金面上项目（81571555）；辽宁省自然科学基金（2015020714） |
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| 摘 要： | 目的：研究HLA-G阳性的胎盘间充质干细在体外诱导Treg的产生。方法：从新生儿胎盘中分离胎盘间充质干细胞的，采用脂质体转染的方式将PEGFP-N1-HLA-G质粒转染到胎盘间充质干细胞中，将细胞分为空白对照组、PEGFP-N1组和PEGFP-N1-HLA-G组，每组设置5个复孔，并通过蛋白质免疫印迹检测HLA-G的表达，将鉴定后的细胞与健康人外周血中CD4+的T淋巴细胞混合培养24 h和48 h，并检测CD4+CD25+Foxp3+Treg占T淋巴细胞的比例。结果：PEGFP-N1-HLA-G转染后胎盘间充质干细胞可以表达HLA-G蛋白，与空白对照组和PEGFP-N1组相比有显著性差异（P<0.01）；HLA-G阳性的胎盘间充质干细胞在与CD4+的T淋巴细胞混合淋巴细胞培养24 h后，Treg细胞占全部T淋巴细胞的比例为（16.41±0.94）%，在培养48 h后，Treg细胞的占全部T淋巴细胞的比例为（16.46±0.59）%，与空白对照组和PEGFP-N1组相比有显著性差异（P<0.01）。结论：HLA-G基因修饰后胎盘间充质干细胞能够有效的在体外诱导CD4+ CD25+ FoxP3+Treg产生。
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| 关 键 词： | HLA-G 胎盘间充质干细胞 T淋巴细胞 调节性T淋巴细胞 |
| 收稿时间： | 2017-07-27 |
Placenta-derived mesenchymal stem cells with HLA-G positive expression induce Treg in vitro |
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| CUI Gui-yu,BAI Jian,MIAO Lan-ying,LIN Da-yong,LIU Hong,LI Ya-li,LIU Xi-cheng.Placenta-derived mesenchymal stem cells with HLA-G positive expression induce Treg in vitro[J].Chinese Journal of Applied Physiology,2018,34(5):396-400. |
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| Authors: | CUI Gui-yu BAI Jian MIAO Lan-ying LIN Da-yong LIU Hong LI Ya-li LIU Xi-cheng |
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| Affiliation: | 1. Medical School of Inner Mongolia University for Nationalities, Tongliao 028000;
2. Liao Ning University of Traditional Chinese Medicine, Shenyang 110847;
3. Department of Anesthesiology, Shenzhen People's Hospital, Shenzhen 518020;
4. Shenzhen Anesthesiology Engineering Center, Shenzhen 518020, China |
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| Abstract: | Objective: To study placenta-derived mesenchymal stem cells with HLA-G (Human Leukocyte Antigen, HLA-G) positive expression induce Treg (regulatory T cell, Treg) in vitro. Methods: placenta-derived mesenchymal stem cells were separated from neonatal placenta; PEGFP - N1 -HLA-G plasmid was transfected in placenta-derived mesenchymal stem cells by liposome transfection.The cells were divided into 3 groups including control group, PEGFP-N1 group and PEGFP-N1-HLA-G group, 5 complex walls in each group. Expression of HLA-G protein was detected by Western Blotting; after identification of cells, healthy human peripheral blood CD4+ T lymphocytes were cultured with placenta-derived mesenchymal stem cells with HLA-G positive expression, and the ratio of CD4+CD25+Foxp3+Treg in T lymphocytes was accounted. Results: After transfection of PEGFP-N1-HLA-G, the placenta-derived mesenchymal stem cells can express HLA-G protein significantly, compared with the control group and PEGFP - N1 group (P<0.01). After HLA-G positive placenta-derived mesenchymal stem cells and CD4 + T lymphocytes were cultured for 24 h, the ratio of CD4+CD25+Foxp3+Treg in T lymphocytes was (16.41±0.94)%. After HLA - G positive placenta-derived mesenchymal stem cells and CD4+ T lymphocytes were cultured for 48 h, the ratio of CD4+CD25+Foxp3+Treg in T lymphocytes was (16.46±0.59)% significantly, compared with the control group and PEGFP - N1 group (P<0.01). Conclusion: Placenta-derived mesenchymal stem cells modified by HLA-G gene can effectively induce CD4+CD25+Foxp3+Treg in vitro. |
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| Keywords: | human leucocyte antigen-G placenta-derived mesenchymal stem cell T lymphocyte regulatory T lymphocyte |
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