Epstein-Barr virus can establish infection in the absence of a classical memory B-cell population |
| |
| Authors: | Conacher Margaret Callard Robin McAulay Karen Chapel Helen Webster David Kumararatne Dinakantha Chandra Anita Spickett Gavin Hopwood Paul A Crawford Dorothy H |
| |
| Affiliation: | Basic and Clinical Virology, University of Edinburgh, Royal (Dick) School of Veterinary Studies, UK. |
| |
| Abstract: | Epstein-Barr virus (EBV) is a ubiquitous human herpesvirus that persists in the body for life after primary infection. The primary site of EBV persistence is the memory B lymphocyte, but whether the virus initially infects na?ve or memory B cells is still disputed. We have analyzed EBV infection in nine cases of X-linked hyper-immunoglobulin M (hyper-IgM) syndrome who, due to a mutation in CD40 ligand gene, do not have a classical, class-switched memory B-cell population (IgD(-) CD27(+)). We found evidence of EBV infection in 67% of cases, which is similar to the infection rate found in the general United Kingdom population (60 to 70% for the relevant age range). We detected EBV DNA in peripheral blood B cells and showed in one case that the infection was restricted to the small population of nonclassical, germinal center-independent memory B cells (IgD(+) CD27(+)). Detection of EBV small RNAs, latent membrane protein 2, and EBV nuclear antigen 3C expression in peripheral blood suggests full latent viral gene expression in this population. Analysis of EBV DNA in serial samples showed variability over time, suggesting cycles of infection and loss. Our results demonstrate that short-term EBV persistence can occur in the absence of a germinal center reaction and a classical memory B-cell population. |
| |
| Keywords: | |
| 本文献已被 PubMed 等数据库收录！ |
|
