Gated conductances in native and reconstituted membranes from frog olfactory cilia. |
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| Authors: | T Nakamura H H Lee H Kobayashi and T O Satoh |
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| Affiliation: | Department of Applied Physics and Chemistry, University of Electro-Communications, Tokyo, Japan. |
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| Abstract: | Although cAMP is well established as a second messenger for olfactory transduction in vertebrates, the role of inositol 1,4,5-trisphosphate (IP3) in this process remains controversial. We addressed this issue by comparing currents evoked by cAMP and IP3 in native and reconstituted membranes from olfactory cilia. We detected only a cyclic nucleotide-gated conductance in the native membrane but both cyclic nucleotide-gated and IP3-gated conductances in the reconstituted membrane. The magnitudes of the cyclic nucleotide- and IP3-gated conductances were not correlated with each other in reconstituted membranes, suggesting that cyclic nucleotide- and IP3-gated channels originate in different cellular compartments. |
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