Characterization and genetic diversity of Pseudomonas syringae isolates from stone fruits in north‐western Iran |
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| Authors: | Fardin Nosratnezhad Kiomars Rouhrazi Nabi Khezrinezhad |
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| Affiliation: | 1. Department of Agriculture, Malekan branch, Islamic Azad University, Malekan, Iran;2. Department of Plant Protection, Mahabad Branch, Islamic Azad University, Mahabad, Iran |
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| Abstract: | From 33 Iranian fluorescent Pseudomonas isolates originating from symptomatic tissues of peach (Prunus persica), plum (Prunus domestica), sweet (Prunus avium) and sour cherry (Prunus cerasus), 27 were identified as Pseudomonas syringae using LOPAT tests. Further characterization of those isolates by GATTa and L‐lactate utilization tests and the detection of syringomycin and coronatine and yersiniabactin coding genes showed that five of them belonged to race 1 and four to race 2 of P. syringae pv. morsprunorum (Psm) and eighteen other isolates were identified as P. syringae pv. syringae (Pss). Based on the analysis of the fingerprint patterns generated by REP, ERIC and BOX‐PCR, the strains were differentiated into three main groups at the 67% similarity level. Strains of the groups 1, 2 and 3 belong to Psm race 1, Psm race 2 and Pss, respectively. Rep‐PCR analysis showed high intra‐pathovar variation within the Pss isolates, which grouped into four distinct clusters. Using the REP primers, the percentage of polymorphic loci was 74.61%, whereas with BOX and ERIC primers, it was 60.5 and 55.21%, respectively. Finally, this study is the first report of the isolation of P. syringae pv. morsprunorum race 1 and 2 strains from stone fruit trees in Iran. |
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| Keywords: | Iran Pseudomonas syringae pv morsprunorum Pseudomonas syringae pv syringae Rep‐PCR Stone fruit trees |
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