Surface protein IsdC and Sortase B are required for heme-iron scavenging of Bacillus anthracis |
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| Authors: | Maresso Anthony W Chapa Travis J Schneewind Olaf |
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| Affiliation: | Department of Microbiology, University of Chicago, Chicago, IL 60637, USA. |
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| Abstract: | Bacillus anthracis, the spore-forming agent of anthrax, requires iron for growth and is capable of scavenging heme-iron during infection. We show here that the B. anthracis iron-regulated surface determinants (isd) locus encompasses isdC, specifying a heme-iron binding surface protein. Anchoring of IsdC to the cell wall envelopes of vegetative bacilli requires srtB, which encodes sortase B. Purified sortase B cleaves IsdC between the threonine and the glycine of its NPKTG motif sorting signal. B. anthracis variants lacking either isdC or srtB display defects in heme-iron scavenging, suggesting that IsdC binding to heme-iron in the cell wall envelope contributes to bacterial uptake of heme. |
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