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Protein‐based nanoformulations for α‐tocopherol encapsulation |
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| Authors: | Fatemeh Shahmoradi Ghaheh Akbar Khoddami Farzaneh Alihosseini Andreia Gomes Artur Ribeiro Artur Cavaco‐Paulo Carla Silva |
| Affiliation: | 1. Department of Textile Engineering, Isfahan University of Technology, Isfahan, Iran;2. CBMA (Centre of Molecular and Environmental Biology), Department of Biology, University of Minho, Campus of Gualtar, Braga, Portugal;3. Centre of Biological Engineering, University of Minho, Campus of Gualtar, Braga, Portugal |
| Abstract: | Nanoparticles of BSA and silk fibroin (SF) with entrapped α‐tocopherol were produced via ultrasonic emulsification. Populations with particle size of 200–300 nm and highly negatively charged were obtained for all the tested formulations. Entrapment efficiencies of around 99% revealed the effective encapsulation of α‐tocopherol into the produced nanoformulations. Generally, these nanodevices did not induce significant cytotoxicity to human skin keratinocytes for all the concentrations tested. The developed formulations showed free radical scavenging of ABTS.+ ability resulting from the synergistic effect between the proteins in formulation and the entrapped tocopherol. Overall, the results contribute for the establishment of BSA:VO and BSA:SF:VO as biodegradable and non‐toxic nanoformulations for the functionalization of textile devices and controlled delivery of tocopherol into the skin. |
| Keywords: | α ‐Tocopherol Anti‐oxidant activity Protein‐based nanoparticles Skin treatment Ultrasounds |
