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Isolation of Temperature-Sensitive Membrane Mutants of Escherichia coli K-12 |
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| Authors: | Johan F Steenbakkers J H F F Broekman A Kerkenaar and P G De Haan |
| Abstract: | Mutants with impaired biosynthesis of unsaturated fatty acids or altered metabolism of the phospholipids were isolated at a rather high frequency from a set of temperature-sensitive lysis mutants. It is suggested that preselection for the lysis phenotype makes it possible to isolate several kinds of mutants affected in the integrity of the cytoplasmic membrane. |
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