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高效液相色谱法检测发酵液中木糖和木糖醇
引用本文:方宏,曾健智,张厚瑞.高效液相色谱法检测发酵液中木糖和木糖醇[J].广西植物,2004,24(3):275-277,198.
作者姓名:方宏  曾健智  张厚瑞
作者单位:中国科学院,广西植物研究所,广西桂林,541006
基金项目:广西自然科学基金资助项目 (98110 15 )
摘    要:建立高效液相色谱检测发酵液中木糖和木糖醇含量的分析方法。色谱柱为HypersilNH2 柱 (4 .6mmi.d.× 2 5 0mm ,5 μm) ,柱温 3 5℃ ,流动相为乙腈—水 (80∶2 0 ) ,流速 1 .0mL .min 1,示差折光检测器检测。木糖和木糖醇在 3 .0~ 60mg.mL 1范围内 ,峰面积与其浓度线性关系良好 (г=0 .9995 ) ;平均回收率分别为 96.0 7% (n =5 ,RSD =0 .5 1 % )和 97.47% (n =5 ,RSD =1 .1 3 % )。方法简便、快速、准确。

关 键 词:木糖  木糖醇  发酵  高效液相色谱
文章编号:1000-3142(2004)03-0275-03

Determination of xylose and xylitol in fermentation broth by high performance liquid chromatography
FANG Hong,ZENG Jian-zhi,ZHANG Hou-rui.Determination of xylose and xylitol in fermentation broth by high performance liquid chromatography[J].Guihaia,2004,24(3):275-277,198.
Authors:FANG Hong  ZENG Jian-zhi  ZHANG Hou-rui
Abstract:A method for the determination of xylose and xylitol in fermentation broth by high performance liquid chromatography is described. The operating conditions were Hypersil NH_2 column(4.6 mm i.d.×250 mm,5 μm)at 35 ℃ and differential refractive index detector. Mobile phase was acetonitrile-water(80∶20;volume ratio)and the flow-rate was 1.0 mL.min~(-1). The linear range was 3.0 mg.mL~(-1) to 60.0 mg.mL~(-1),the correlation coefficient was 0.999 5. The corresponding average recoveries were 96.07%(n=5,RSD=0.51%)and 97.47%(n=5,RSD=1.13%)respectively. The method is simple,rapid and accurate.
Keywords:xylose  xylitol  fermentation process  high performance liquid chromatography
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