The SHIrelated sequence (SRS) gene family regulates plant flowering, growth and development by mediating hormonal changes, and plays an important regulatory role in adaptation to environmental stress. Based on the apple (Malus domestica Borkh.) genome data, this study identified the members of the apple SRS gene family by bioinformatics, and analyzed the characteristics, functions and expression of the conserved domains of the SRS gene family. The results showed that: (1) the MdSRS gene family consisted of 11 members, named MdSRS1-MdSRS11, which were unevenly distributed on 9 apple chromosomes. (2) The MdSRS proteins contain 229-414 amino acid residues, and the isoelectric point distribution are between 6.38 and 9.36, and the subcellular localization results show that the MdSRS proteins are mostly distributed in the cell membrane, and also in the nucleus and chloroplast. (3) Phylogenetic analysis by introducing SRS genes from Arabidopsis, rice, tomato and poplar showed that 11 MdSRSs were divided into 5 subfamilies (A₁-A₅), with the most distribution in A4. (4) The analysis of cisacting elements showed that the 2 000 bp sequences upstream of the 11 MdSRS promoters were distributed with response elements such as hormones, environmental adaptability and stress induction. (5) The results of realtime quantitative PCR showed that the overall expression was downregulated under salt stress and drought stress, and most of them were upregulated after ABA stress. It is a potential resistance candidate gene, indicating that the SRS family is ABAregulated and other abiotic genes. Stress has a regulatory effect. The research shows that 11 members of the SRS family are involved in regulating the response to various adversities such as drought, salt and ABA stress, providing a theoretical basis for apple to withstand adverse environments.