Carotenoid Cleavage Dioxygenase (CCD) is a key enzyme in the oxidative cleavage pathway of carotenoids, and plays an important role in plant growth and development, aroma formation and stress response. In this study, 13 CCD genes with complete RPE65 conserved domain were identified from the whole genome of zucchini by bioinformatics method. The above results provides a reference for the subsequent analysis of the function of CCD gene family in zucchini. The results showed that: (1) cluster analysis showed that 13 CCD genes encoding proteins of zucchini could be divided into six subgroups: CCD1, CCD4, CCD7, CCD8, NCED and CCD1like, then CCD7and CCD8 subgroups have a long genetic distance from other family members. (2) Cis acting element prediction analysis found that the CCD gene promoters contained light signal, hormone, environmental stress and growth and development response elements. (3) Transcriptome data analysis showed that CCD family genes had tissuespecific expression, and three CCD genes were not expressed in tissues, while CpCCD1 was highly expressed in leaves and fruits. (4) During fruit development, eight CCD genes were upregulated and two CCD genes were down regulated, and four CCD genes, CpCCD1, CpCCD4a, CpCCD4b and CpCCD8a were significantly high expression in fruit expanding growth and maturation, suggesting that they may play an important regulatory role in fruit development of zucchini.