In order to study elucidate the relationship between fertility development and endogenous hormones in thermo-photo-sensitive male sterile wheat line BNS,we toke leaves,young panicles and anthers from BNS fertile plants and sterile plants as experimental materials (fresh weight) in this study and they were collected through the stage of pistil and stamen differentiation (Ⅰ) to the trinuclear stage (Ⅵ).And the contents of auxin,abscisic acid and gibberellin in leaves,young panicles and anthers from sterile and fertile lines were detected using indirect ELISA methods.The results are as follows:(1)As the sterile line development,the hormones changes were different from those in fertile line.The IAA contents in sterile and sterile lines were 33~95 ng/g and 57~112 ng/g respectively through the development stages.The IAA contents in ferile line were lower than those in fertile line (P<0.05).The contents of ABA and GA in sterile line displayed irregular changes compared with those in fertile line.The contents of ABA and GA in sterile line were 48~129 ng/g and 3~14 ng/g respectively in sterile line,and those in fertile were 77~132 ng/g and 4~11 ng/g,respectively.(2)At pistil and stamen differentiation stage,the contents of IAA and GA in young panicles from sterile line were 33.85 ng/g and 7.13 ng/g,and those from the fertile line were 50.55 ng/g and 11.84 ng/g,respectively.The contents of the respective hormones from different lines were highly significant differences (P<0.01).(3)Through the development stages,the ratios of IAA/ABA,IAA/GA and ABA/GA in sterile line were 0.4~1.1,4.3~15 and 7~20 respectively,and those in fertile were 0.5~0.9,4.3~11.5 and 7.3~13 respectively.Compared with those in fertile line,the ratios of hormones in sterile line displayed more changeable and were be out of proportion through the developmental stages.These results indicated the unbalanced ratios of hormones might be one of the causes that lead to male sterility,more specially,the decreased levels of IAA and GA in pistil and stamen differentiation stage might promote the occurrence of male sterility in BNS.