The nontoxic fragment C of tetanus toxin(TTC) can transport other proteins from the circulation to central nervous system motor neurons. Increased levels of CuZn-SOD are protective in experimental models of stroke and Parkinsons's diseases, where mutations in SOD may cause motor neuron disease. Here the human Mn-SOD is linked to tetanus toxin fragment C gene to construct the fusion gene, then was ligated into prokaryotic expression vector pET-22b（+），expression of the plasmid in E.coli, resulted in the production of a protein has a subunit molecular mass of 71 ku and is recognized by both anti-Mn-SOD and anti-tetanus toxin antibody. The Mn-SOD moiety retains substantial enzymatic activity，where the TTC moiety can delivers the fusion protein to central nervous system neurons. Such fusions should provide a powerful tool for investigating the protective and destructive roles of Mn-SOD in motor neurons.