The aim of studying on animal model of Parkinson's Disease (PD) is to discover the mechanism underlying the specific lesion of the dopaminergic neurons, then to seek out protective ways or therapies against the lesion. The mouse model of PD induced by neurotoxin 1-methyl-4-phenyl-1,2,3,6-tetrapyridine (MPTP) is applied extensively in the studies of the sporadic PD. Based on differences in the total amount of MPTP administrated, time between injections, and the injection style (intraperitoneally versus subcutaneously), various models of PD, which could satisfy many different studies, have been created. The assessment of the MPTP lesion is multilevel and contains a series of parameters. The origin of MPTP-lesioned model of PD and the pathways of MPTP causing lesions to dopaminergic neurons were systematically illuminated, then the creation and assessment of MPTP-lesioned PD model was elaborated.