Wnt signaling is implicated in the control of cell growth and differentiation during neural stem cell(CNS) development.Wnt3a, one of wnt gene family members, has effect on regeneration neurospheres and differentiation into neurons.Wnt3a inhibits regeneration of neurospheres, and promotes its differentiation. In vitro neurosphere was cultured in a serum-free defined medium DMEM/F12 supplemented with bFGF and EGF. Dissociated cells were plated onto poly-d-lysine-coated coverslips and propagated in medium containing recombined Wnt3a-adenovirus. Plenty of Nurr1 were detected by RT-PCR after 3 days. Wnt3a combined AA would improve NSC differentiation into dopaminergic (DA) neuron. The quantity of DA neuron is obviously more than the AA alone group's. Moreover, the expression of TH mRNA is 1.86 fold in Wnt3a combined AA group. Induced cells were immunostained for TH and DAT. The proportion of TH-positive was (37.42±2.54) % (P<0.05) in the AA group , accordingly in Wnt3a combined AA group it was (73.96±2.61) % (P<0.05). These results indicate that Wnt3a promoted the proliferation of precursor cells expressing the orphan nuclear receptor-related factor 1 (Nurr1) . Large numbers of DA neurons are obtained by adding AA in the culture of NSC modified Wnt3a in vitro. Therefore, the successful conversion of neural stem cells in vitro resulting in the desired dopaminergic neuronal phenotype, could provide a solution to the problem of limited availability for clinical surgical transplantation therapies, which are currently in progress for the treatment of neurodegenerative diseases such as Parkinson's disease. These findings indicate that Wnt3a is key regulators of proliferation and differentiation of DA precursors.