Teaching quality is directly related to the performance of universities in fostering talents.Being innovative,high-level,and challenging (IHC) is the basic goal of course reform at universities in the new era.It is essential to reform the contents and teaching mode to improve the IHC properties of the existing courses.We first designed the three-dimensional goals of Molecular Biology Experiment teaching and the contents to support these goals.Then,we pinpointed the common points shared by blended teaching and experiment course,and designed the ways of blended teaching for the course.The reformed course contents and teaching mode have enhanced its IHC properties,and achieved good teaching performance.This paper provides a reference for the reform of experiment courses in universities.