Abstract:［Objective］To understand the bacterial diversity isolated from the cysts of Heterodera glycines in the soybean field in Heilongjiang Province．［Methods］Bacteria were isolated from cysts on nutrient agar plates using dilution plate method and further identified by phylogenetic analysis based on 16S rDNA gene sequences． ［Results］Totally 90 bacteria strains with different colony morphology were selected on nutrient agar plate and their phylogenetic features were analyzed based on the partial 16S rDNA sequences． In total 7 genera and 22 species were identified，including 46 strains in Gammaproteobacteria (51.1%)，32 in Firmicutes (35.6%)，10 in Betaproteobacteria (11.1%)，and 2 in Alphaproteobacteria (2.2%)． The dominant bacteria species were Pseudomonas and Bacillus．［Conclusion］There was abundant species diversity of bacteria isolated from cysts Heterodera glycines in Heilongjiang，and these bacteria may play a physical and ecological roles in nematodes．