拮抗枯草芽孢杆菌KC-5的分离鉴定及其发酵优化

doi:10.13523/j.cb.20140314

中国生物工程杂志

2014, Vol. 34

Issue (3): 96-102 DOI: 10.13523/j.cb.20140314

技术与方法

拮抗枯草芽孢杆菌KC-5的分离鉴定及其发酵优化

柳慧丽¹, 李园园², 鞠瑞成², 赵宏涛², 杨清¹

1. 南京农业大学生命科学学院南京 210000;
2. 青岛明月海藻集团有限公司青岛 266400

Isolation, Identification and Fermentation Optimization of Antagonistic Bacillus subtilis KC-5

LIU Hui-li¹, LI Yuan-yuan², JU Rui-cheng², ZHAO Hong-tao², YANG Qing¹

1. College of Life Sciences, Nanjing Agricultural University, Nanjing 210095, China;
2. Qingdao Bright Moon Seaweed Group Co., Ltd., Qingdao 266400, China

全文: PDF(806 KB) HTML

摘要： 为了筛选出对植物病原菌具有拮抗作用的生物防治细菌，采用平板对峙法从蔬菜根际土壤中分离获得一株对多种病原真菌具有抑制作用的枯草芽孢杆菌，并对抑制后的病原菌菌丝进行了观察，结果表明该菌株对尖孢镰刀菌、串珠镰刀菌、层出镰刀菌以及腐皮镰刀菌均具有抑菌活性。通过形态特征、生理生化特征及16S rRNA序列分析，将该菌株鉴定为枯草芽孢杆菌（Bacillus subtilis），并对其发酵培养基进行了优化。

关键词： 枯草芽孢杆菌; 拮抗; 发酵优化

Abstract: In order to screen biocontrol bacteria being antagonistic to some plant pathogens, Bacillus subtilis KC-5 was isolated from the vegetable soil to inhibit a variety of pathogenic fungi with Tablet confrontation France, and the observations about inhibition of mycelia suggested that the strains KC-5 can inhibit the activity of Fusarium oxysporum, Fusarium moniliforme, Fusarium proliferatum and Fusarium solani. By analyzing the morphological, physiological and biochemical characteristics and 16S rRNA sequence, the strain was identified as Bacillus subtilis and its fermentation medium was optimized.

Key words: Bacillus subtilis Antagonism Fermentation optimization

收稿日期: 2013-11-29 出版日期: 2014-03-25

ZTFLH:

Q815

通讯作者: 杨清 E-mail: qyang19@njau.edu.cn

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引用本文:

柳慧丽, 李园园, 鞠瑞成, 赵宏涛, 杨清. 拮抗枯草芽孢杆菌KC-5的分离鉴定及其发酵优化[J]. 中国生物工程杂志, 2014, 34(3): 96-102.

LIU Hui-li, LI Yuan-yuan, JU Rui-cheng, ZHAO Hong-tao, YANG Qing. Isolation, Identification and Fermentation Optimization of Antagonistic Bacillus subtilis KC-5. China Biotechnology, 2014, 34(3): 96-102.

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https://manu60.magtech.com.cn/biotech/CN/10.13523/j.cb.20140314 或 https://manu60.magtech.com.cn/biotech/CN/Y2014/V34/I3/96

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