乳酸菌基因芯片应用研究进展

中国生物工程杂志

2009, Vol. 29

Issue (06): 113-119

综述

乳酸菌基因芯片应用研究进展

包秋华1|孙志宏1|乌日娜1|孟和2|张和平1

1. 内蒙古农业大学乳品生物技术与工程教育部重点实验室
2. 上海交通大学农业与生物学院

Applications Progress of DNA Microarray in Lactic Acid Bacteria

全文: PDF(484 KB) HTML

摘要： 基因芯片技术是上世纪90年代兴起的一种对成百上千甚至上万个基因同时进行检测的新技术，具有高通量、并行化的特点，广泛应用于基因表达谱测定、基因功能预测、基因突变检测和多态性分析等方面。多种乳酸菌基因组全序列以及其大量EST、16S rDNA、16S-23S基因间区和功能基因序列测定的完成，有力地推动了基因芯片技术在乳酸菌研究中的应用。介绍了基因芯片的基本原理及乳酸菌基因芯片在基因表达、种属鉴定等研究中的应用进展，以期更好地利用和开发乳酸菌基因芯片。

关键词： 乳酸菌；基因芯片；基因表达；种属鉴定

Abstract:

DNA microarray technology emerged since 1990s allows a parallel analysis for thousands of genes in a single experiment. With the many strain's whole genome sequenceing, DNA microarray technology as a primary high-throughput tool has been widely applicated in monitoring of gene expression patterns, such as gene function prediction, detection of gene mutations, polymorphism analysis and so on. More attention has been taken on the application of gene chip technology in the research of Lactic Acid Bacteria（LAB）, as for several LAB strains genome sequencing completed and a lots of sequences of EST, 16S/16S-23S rDNA obtained. The basic principle of the gene chip and the application progress of DNA microarray in identification and expression analysis of LAB were introduced in this paper, which would provide reference for further application and exploitation of LAB DNA microarray.

收稿日期: 2009-01-04 出版日期: 2009-07-02

ZTFLH:

Q819

通讯作者: 孟和 E-mail: hepingZhang@imau.deu.cn，menghe@sjtu.edu.cn

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引用本文:

包秋华1,孙志宏1,乌日娜1,孟和2,张和平1. 乳酸菌基因芯片应用研究进展[J]. 中国生物工程杂志, 2009, 29(06): 113-119.

链接本文:

https://manu60.magtech.com.cn/biotech/CN/Y2009/V29/I06/113

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