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中国生物工程杂志

CHINA BIOTECHNOLOGY
中国生物工程杂志  2013, Vol. 33 Issue (8): 38-44    
研究报告     
藏绵羊乳腺溶菌酶基因的克隆、原核表达及抗菌活性研究
李建波1,2, 江明锋1,2, 王永1
1. 青藏高原动物遗传资源保护与利用四川省重点实验室 成都 610041;
2. 西南民族大学生命科学与技术学院 成都 610041
Tibetan Sheep Mammary Gland Lysozyme: Molecular Cloning, Prokaryotic Expression and Its Antibacterial Activity
LI Jian-bo1,2, JIANG Ming-feng1,2, WANG Yong1
1. Sichuan Provincial Key Laboratory of Qinghai-Tibetan Plateau Animal Genetic Resource Conservation and Exploitation, Chengdu 610041, China;
2. College of Life Science and Technology, Southwest University for Nationalities, Chengdu 610041, China
 全文: PDF(7070 KB)   HTML
摘要: 克隆了藏绵羊溶菌酶(LZM, EC 3.2.1.17)基因cDNA 序列,构建重组质粒pET-32a-LZM,并在大肠杆菌BL21中成功表达,重组LZM有一定的抗金黄色葡萄球菌活性。系统进化树分析表明,藏绵羊乳腺LZM与山羊LZM 的亲缘关系最近。实时荧光定量PCR(RT-qPCR)结果表明,藏绵羊乳腺LZM 在乳腺中表达量最大。此结果对LZM的应用有一定价值。
关键词: 藏绵羊LZM克隆序列分析原核表达抗菌活性    
Abstract: The full length of LZM cDNA sequence in mammary gland of Tibetan sheep was cloned into pET-32α, generating a recombinant plasmid pET-32α-LZM which was successfully expressed its protein product in Escherichia coli BL21. The agarose diffusion assay showed that recombinant LZM inhibited the growth of Staphylococcus aureus. Sequence comparison and phylogenetic analysis indicated that LZM gene in mammary gland of Tibetan sheep was most similar to goat. Quantitative Real-Time PCR (qRT-PCR) results showed that LZM mRNA had a highest expression level in the mammary gland. In conclusion, it laid a solid foundation for further study.
Key words: Tibetan sheep    LZM    Clone    Sequence analysis    Prokaryotic expression    Antibacterial activity
收稿日期: 2013-04-07 出版日期: 2013-08-25
ZTFLH:  Q819  
基金资助: 国家科技支撑计划(2012BAD13B06);中央高校基本科研业务费专项资金(11ZYXS24,12ZYXS);四川省科技创新产业链示范工程重大项目(2011NZ0003);四川省2011年度学术技术带头人和留学归国人员项目资助项目
通讯作者: 王永wangyong010101@swun.cn     E-mail: wangyong 010101@swun.cn
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引用本文:

李建波, 江明锋, 王永. 藏绵羊乳腺溶菌酶基因的克隆、原核表达及抗菌活性研究[J]. 中国生物工程杂志, 2013, 33(8): 38-44.

LI Jian-bo, JIANG Ming-feng, WANG Yong. Tibetan Sheep Mammary Gland Lysozyme: Molecular Cloning, Prokaryotic Expression and Its Antibacterial Activity. China Biotechnology, 2013, 33(8): 38-44.

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https://manu60.magtech.com.cn/biotech/CN/        https://manu60.magtech.com.cn/biotech/CN/Y2013/V33/I8/38

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