研究报告 |
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乳酸克鲁维酵母Cu/Zn-SOD基因的克隆及在酿酒酵母中的表达研究 |
李文凤1,2,季静1**,王罡1,牛宝龙1,王海勇1,2 |
1.天津大学农业与生物技术学院 天津 300072
2. 天津大学化工学院 天津 300072 |
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Clone of Cu/Zn-Superoxide Dismutase Gene from Kluveromces lactis and Its expreesion in Saccharomyces cerevisiae |
LI Wen-feng1,2,JI Jing1,WANG Gang1,NIU Bao-long1,WANG Hai-yong1,2 |
1.School of Agriculture and Bioengineering, Tianjin University, Tianjin 300072, China
2.School of Chemical Engineering and Technology, Tianjin University, Tianjin 300072, China |
[1] Fridovich I. Superoxide radical and superoxide dismutases. Annu Rev Biochem, 1995, 64:97112.
[2] Miller A F. Superoxide Processing. In Coordination Chemistry in the Biosphere and Geosphere. Edited by Que L Jr, Tolman W. Oxford, Amsterdam, New York and Tokyo: Pergamon, 2003. 479506.
[3] Raimondi S, Uccelletti D, Matteuzzi D, et al. Characterization of the superoxide dismutase SOD1 gene of Kluyveromyces marxianus L3 and improved production of SOD activity. Appl Microbiol Biotechnol, 2008, 77:12691277.
[4] Ping Y. Enhancing survival of Escherichia coli by increasing the periplasmic expression of Cu,Zn superoxide dismutase from Saccharomyces cerevisiae. Appl Microbiol Biotechnol, 2007, 76:867871.
[5] Badawi G H, Yamauchi Y, Shimada E, et al. Enhanced tolerance to salt stress and water deficit by overexpressing superoxide dismutase in tobacco (Nicotiana tabacum) chloroplasts. Plant Science, 2004, 166:919928.
[6] Cabiscol E, Ros J, in: DalleDonne I, Scaloni A, Butterfield D A. Oxidative Damage to Proteins: Structural Modifications and Consequences in Cell Function, Redox Proteomics.Hoboken New Jersey: Wiley Interscience, 2006.247281.
[7] Marklund S. Involvement of the superoxide anion radical in the autoxidation of pyrogallol and a convenient assay for superoxide dismutase. Eur J Biochem, 1974, 47:469474.
[8] Gralla E B, Valentine J S. Null mutants of Saccharomyces cerevisiae Cu/Zn superoxide dismutase: characterization and spontaneous mutation rates, J Bacteriol, 1991, 173:59185920.
[9] Scandalios J G. The rise of ROS. Trends in biochemical. Sciences, 2002, 27:483486.
[10] Angelova M B. Heatshockinduced oxidative stress and antioxidant response in Aspergillus niger 26. Canadian Journal of Microbiology, 2008, 54(12):977983.
[11] Reddy M V, Gangadharam PR. Heat shock treatment of macrophages causes increased release of superoxide anion. Infect Immun, 1992, 60:23862390.
[12] Hae Yong Yoo, Shin Seog Kim, Hyune Mo Rho. Overexpression and simple purification of human superoxide dismutase (SOD1) in yeast and its resistance to oxidative stress. J Biotechnol, 1999, 68:2935. |
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