Abstract: To investigate the effect of embryo transfer in buffaloes, buffaloes of Yunnan were experimented in 2002. Results showed:(1)Synchronous estrus and usable rates of donors and recipients administered with cloprostenol (PG) 0.6 mg/head,injection once,were 43.33% (13/30) and 16.67% (5/30). Especially,the synchronous estrus rate was higher in paritys than in heifer s (P=0.086),higher in crossbreds than in Dehong Buffalos (P=0.153),and higher in weight from 401 to 530 kg of buffalo cows than from 300 to 400 kg (P<0.05);And the usable rate of buffaloes with evidence estrus behavior was also higher than that of buffaloes with lack of evidence estrus (P<0.01).(2)Five donors crossbred F1 (Murrah×Dehong buffalo) were picked out from buffaloes of synchronous estrus and divided into two groups,I.e. import hormone group (n=2) and native hormone group (n=3),which were dealt with superovulation. The results showed that 2 out of 5 buffaloes gave 9 embryos,and average no. of recovered embryos and no. of transferable embryos in the import hormone were 2.0 and 1.5,and higher than those in the native hormone,which were 1.67 (P=0.454) and 0.33 (P=0.288),respectively.(3)Four fresh embryos were transferred into 3 receipts,thereinto the rate of 90-day conception was 33.33%,but finally none gave birth to a calf. The experiment results indicate that it is in effect for superovulation of buffaloes with administered 24 mg of import FSH and plus PG (LutalyseR) 35 mg and the same as 11 mg of native FSH and plus PG 0.8 mg,meanwhile suggest that it is practicable if the receipt buffaloes are enough for synchronous estrus,then the cows with evidence estrus behavior and good CL checked can be selected for transferring embryos.