To study the chemical components and the antifungal activity of extraction from conidia of Trichoderma viride LTR-2. The extraction were obtained by distilling with Methylene dichloride from conidia of Trichoderma viride LTR-2 cultured on wheat bran solid matrix. Antifungal activity were determined by mycelium growth method. The chemical components of the extraction were analysed by GC-MS, the relative components in the extraction were determined by area normalization. The extraction not only have broad-spectrum control, showed antibiosis against eleven different plant fungal pathogens in PDA dish, such as Rhizoctonia solani, Alternaria brassica, Verticillium dahliae, Macrophoma kawatsukai, Fusarium moniliforme, Botrytis cinerea, Rhizoctonia cerealis, Fusarium oxysporum f.sp.vasinfectum, Bipolaris sorokinana, Fusarium graminearum, Alternaria.mali, but also have high inhibitory effect, and had 89.3% suppressive rate to Rhizoctonia cerealis. About sixty components were separated and identified by GC-MS, majority components were Hydrocarbon, the number of the Hydrocarbon were fourty-three kinds. Ergosterol was the major chemical components of the extract, and has 41.90% content. Other components comprised: Ketone, Organic acid, Alcohol, Ene, et al. Conclusion: The extraction from conidia of Trichoderma viride LTR-2 have antifungal activity. The extration comprised 2H-Pyran-2-one, 5, 6-dihydro-6-pentyl, it has 2.35% content. reference others literature, 2H-Pyran-2-one, 5, 6-dihydro-6-pentyl may be the suppressive component of the extration.