The symbiotic seed germination of Pleione bulbocodioides (Franch.) Rolfe, a traditional Chinese medicine, was investigated. The results showed that the mycorrhizal fungi isolated from pleione bulbocodioides, GN21, GN23 and GN24 promoted the seed germination evidently, and the germination rate of GN21 was up to 84.6%, much better than the others and control. However, the fungal species isolated from the same species of orchid, even if belonging to the same genus, could not be always to stimulate the seed germination, some species even became pathogens under the circumstances of poor nutrition.