Various samples of Douchi, a kind of traditional Chinese fermented soybean food, were collected from provinces of China. Different strains with fibrinolytic activity were screened from these samples by enrichment culture medium and fibrin plate was used to confirm their fibrinolytic activity. Thirteen distinct strains were identified as Bacillus sp., Streptomyces sp., Pseudomonas sp. and Arthrobacter sp. by classical classification approaches, chemical approaches and 16S rDNA sequencing. Nine Dou chi fibrinolytic enzyme-producing strains were gained.