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氯化钠胁迫对嫁接茄子生长、抗氧化酶活性和活性氧代谢的影响

刘正鲁;朱月林;胡春梅;魏国平;杨立飞;张古文   

  1. 南京农业大学园艺学院, 南京 210095
  • 收稿日期:2006-04-11 修回日期:2006-12-30 出版日期:2007-03-18 发布日期:2007-03-18

Effects of NaCl stress on the growth, antioxidant enzyme activities and reactive oxygen metabolism of grafted eggplant

LIU Zheng-lu; ZHU Yue-lin; HU Chun-mei; WEI Guo-ping; YANG Li-fei; ZHANG Gu-wen   

  1. College of Horticulture, Nanjing Agricultural University, Nanjing 210095, China
  • Received:2006-04-11 Revised:2006-12-30 Online:2007-03-18 Published:2007-03-18

摘要: 以日本引进的茄子设施栽培专用耐盐品种‘Torvum Vigor’为砧木,栽培品种‘苏崎茄’为接穗,在营养液栽培条件下,对80 mmol·L-1 NaCl胁迫下茄子嫁接苗和自根苗的光合特性、叶片抗氧化酶活性、活性氧代谢进行了比较.结果表明:NaCl胁迫下,茄子嫁接苗的干质量、叶绿素含量和光合速率分别比苏崎茄自根苗高67。8%、8。8%和31。1%,均有显著差异;抗氧化酶活性均显著高于苏崎茄自根苗,而O2-·生成速率、H2O2和MDA含量则显著低于苏崎茄自根苗.NaCl胁迫明显降低了叶片叶绿素含量和光合速率,显著增加了O2-·生成速率、H2O2和MDA含量,但嫁接苗受NaCl胁迫的影响小于苏崎茄自根苗,嫁接苗较强的耐盐性与NaCl胁迫下较高的抗氧化酶活性和较低的氧化损伤有关.

关键词: 大豆, 土壤有效硅, 种子萌发, 幼苗生长, 生理功能

Abstract: By the method of hydroponics and with the salt-tolerant eggplant cultivar ‘Torvum Vigor’(Solanum torvum) from Japan as rootstock and the cultivar ‘Suqiqie’ (Solanum melongena L.) as scion, this paper studied the differences between grafted and own-root seedlings in their photosynthetic characteristics, antioxidant enzyme activities, and reactive oxygen metabolism under 80 mmol·L-1 NaCl stress. The results showed that under NaCl stress, the dry mass, chlorophyll content and photosynthetic rate of grafted seedlings were 67.8%, 8.8% and 31.1% higher than those of own-root seedlings, respectively, and the antioxidant enzyme activities were significantly higher while the O2-· producing rate and the hydrogen peroxide (H2O2) and malondiadehyde (MDA) contents were significantly lower in grafted seedlings leaves than in own-root seedlings. For both grafted and own-root seedlings, NaCl stress reduced the chlorophyll content and photosynthetic rate, but increased the O2-· producing rate and the H2O2 and MDA contents significantly. However, the growth of grafted seedlings was less affected by NaCl stress. The stronger salt tolerance of grafted eggplant seedlings was related to their higher antioxidant enzyme activities and less oxidative damage.

Key words: Soybean, Available silicon in soil, Seed germination, Seedlings growth, Physiological function