镉胁迫对大弹涂鱼肝脏黄螵呤氧化酶和抗氧化酶活性的影响

应用生态学报

镉胁迫对大弹涂鱼肝脏黄螵呤氧化酶和抗氧化酶活性的影响

刘伟成¹;李明云¹;黄福勇^1,2;竺俊全¹;董武³;杨景峰³

¹宁波大学生命科学与生物工程学院，宁波 315211;²浙江省水产质量检测中心，杭州 310012；³内蒙古民族大学动物科技学院，通辽 028042

收稿日期:2005-07-11 修回日期:2006-05-16 出版日期:2006-07-18 发布日期:2006-07-18

Effects of cadmium stress on xanthine oxidase and antioxidant enzyme activities in Boleophthalmus pectinirostris liver

LIU Weicheng¹; LI Mingyun¹; HUANG Fuyong^1,2; ZHU Junquan¹; DONG Wu³; YANG Jingfeng³

¹ Faculty of Life Science and Biotechnology, Ningbo University, Ningbo 315211, China; ²Zhejiang Provincial Fisheries Quality Detection Center, Hangzhou 310012, China; ³College of Animal Science and Technology, Inner Mongolia University for Nationalities, Tongliao 028042, China

Received:2005-07-11 Revised:2006-05-16 Online:2006-07-18 Published:2006-07-18

摘要/Abstract

摘要： 研究了不同浓度镉离子对大弹涂鱼肝脏黄嘌呤氧化酶（XOD）、抗氧化酶（超氧化物岐化酶SOD、过氧化氢酶CAT）活性和丙二醛（MDA）含量的影响，以探讨其用于污染暴露的生物标记的可行性.结果表明，低浓度Cd²⁺（0.05 mg·L^-1）暴露使大弹涂鱼肝脏XOD和SOD活性随时间延长升高，第10天达到最大值，中高浓度暴露（0.5 和5 mg·L^-1Cd²⁺）XOD和SOD活性显著或极显著升高；低和高浓度镉胁迫处理的CAT活性在12 h显著降低，随时间的延长低浓度组CAT活性恢复正常，高浓度组在第7天降到最低值, 并在恢复期的5 d中高浓度组CAT活性却极显著升高;低和中浓度镉胁迫处理的MDA含量12 h极显著升高，而高浓度却极显著下降，随时间延长低浓度恢复正常, 中浓度先上升后下降并到第5天达到最大值，而中高浓度在恢复5 d后MDA含量都极显著降低.

Abstract: This paper approached the feasibility of using the xanthine oxidase (XOD), superoxide dismutase (SOD) and catalase (CAT) activities and malonyldialdehyde (MDA) content in Boleophthalmus pectinirostris liver as the indicators of Cd²⁺ toxic effects on this marine fish. Three concentrations of cadmium chloride (0.05, 0.5 and 5 mg Cd²⁺·L^-1) were enacted. The results showed that in treatment 0.05 mg Cd²⁺·L^-1, the XOD and SOD activities in B. pectinirostris liver increased with time and reached the maximum on the 10th day, while in treatments 0.5 and 5 mg Cd²⁺·L^-1, they increased significantly or extremely significantly, compared with the control. The CAT activity in treatments 0.05 and 5 mg Cd²⁺·L^-1decreased significantly within 12 hours, but recovered then in treatment 005 mg Cd²⁺·L^-1. After moved into clean seawater for 5 days, the CAT activity increased extremely significantly in treatments 0.5 and 5 mg Cd²⁺·L^-1, but reversed in treatment 0.05 mg Cd²⁺·L^-1. As a product of antioxidation and oxidation, the content of MDA in treatments 0.5 and 5 mg Cd²⁺·L^-1 decreased extremely significantly after recovered for 5 days. It was suggested that the XOD and SOD activities in B. pectinirostris liver could be used as a biomarker of Cd²⁺ pollution, while the CAT activity and MDA content in this marine fish liver were not sensitive to Cd²⁺ stress.

Key words: Nitrogen, Calcium, Polyphenol oxidase, Peroxidase, Carnation, Leaf spot

刘伟成¹;李明云¹;黄福勇^1,2;竺俊全¹;董武³;杨景峰³. 镉胁迫对大弹涂鱼肝脏黄螵呤氧化酶和抗氧化酶活性的影响[J]. 应用生态学报.

LIU Weicheng¹; LI Mingyun¹; HUANG Fuyong^1,2; ZHU Junquan¹; DONG Wu³; YANG Jingfeng³. Effects of cadmium stress on xanthine oxidase and antioxidant enzyme activities in Boleophthalmus pectinirostris liver[J]. Chinese Journal of Applied Ecology.