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| 瑞拉菌素产生菌GAO-1-GR-2发酵工艺条件优化研究 |
| Optimization of Fermentation Technology Conditions for Zuelamycin-Producing Strain GAO-1-GR-2 |
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| DOI： |
| 中文关键词: 链霉菌 发酵 农用抗生素 瑞拉菌素 效价 |
| 英文关键词: Streptomyces fermentation agricultural antibiotics zuelamycin valence |
| 基金项目:国家高技术研究发展计划 |
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| 摘要点击次数: 52 |
| 全文下载次数: 39 |
| 中文摘要: |
| 对瑞拉菌GAO-1-GR-2的发酵条件进行了单因素研究，通过正交试验设计对该菌株的培养基进行了筛选。并对其发酵条件进行了研究，结果表明：有机氮源以酵母粉和黄豆饼粉为宜；碳源以葡萄糖和饴糖最好，但考虑到发酵成苓，应选用葡萄糖2％。发酵培养基应呈中性，以采用自然pH为宜，pH超过8，或小于7，则会抑制发酵产物活性；通气量为1：0．5最好，能降低20％耗氧量；摇床转速140r／min，培养温度30℃，发酵周期72h。抗生素发酵原粉效价能达到4000μg／mL。 |
| 英文摘要: |
| The fermentation conditions of strain Ruila GAO-1-GR-2 were studied with single factor, and then its me-dium was screened through orthogonal experiment design. The results showed that yeast powder and soybean meal were suitable for organic nitrogen sources; glucose and maltose were the best carbon sources; however, taking the cost of fermentation into consideration, 2% glucose was the best alternative. And the medium should be neutral, and adopt it at natural pH; pH over 8 or below 7 will inhibit the activity of fermentation product. The optimal ventilation was at 1 : 0.5 which could reduce 20% of oxygen consumption; rpm of shaker was at 140, culture temperature at 30 ℃, with fermentation period for 72 hours. With these conditions the valence of the original powder of fermentation could reach up to 4 000 μg/mL. |
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