| This study conducted laboratory testing and diagnostic analysis on 3 imported cases of quartant malaria from abroad in Liaoning Province in 2019. Collected and conducted epidemiological investigations and data gathering. According to the requirements of the latest diagnostic standard "Malaria Diagnosis" (WS259-2015) currently implemented by the malaria laboratory, the collection of anticoagulant blood for clinically diagnosed patients with suspected quartant malaria requires blood smear microscopy and rapid malaria diagnostic testing (RDT) and send whole blood to Liaoning Provincial Center for Disease Control and Prevention for case review, Nested PCR detection and sequencing comparison. The thin and thick blood film was microscopically examined by peripheral blood smears of 3 patients. The stages of the worms were mainly ring shaped stage, macrotrophozoite stage, gametophyte stage and mature schizont stage. In the macrotrophozoic stage, malaria pigment was dark brown, larger, distributed along the edge, at the same time, it can be found that the parasitic red blood cells were usually not swollen or even reduced. The gametophyte was small and round. According to the characteristics of the microscope, it was preliminarily judged to be Plasmodium malariae. The RDT result indicated that the infection was not malignant. For single infections of three other malarias (quartant malaria, oval malaria, vivax malaria) except malaria, the provincial reference laboratory used Nested PCR detection technology to check the whole blood; The target fragment product sequence of the quartant malaria was sent to Shanghai Wikigene Sequencing Company for sequence analysis and comparison, and the gene sequence homology reached 100%. At the same time, blood smear microscopy, rapid malaria diagnostic test (RDT) and PCR were used for detection, and the experimental results were all identified as three quartant malaria cases. At the same time, according to the epidemiological investigation and clinical manifestations of the cases, they were identified as imported cases of three quartant malaria cases. |
