| 李晓莉 赵艳滨 孙立春 李乐静.Id 基因在多种肺癌细胞中的表达及意义[J].,2006,6(2):36-38 |
| Id 基因在多种肺癌细胞中的表达及意义 |
| The expression and significance of Id in different lung cancer cells |
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| DOI： |
| 中文关键词: 肺癌 Id 基因 癌基因 |
| 英文关键词: Lung cancer Id gene Oncogene |
| 基金项目: |
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| 中文摘要: |
| 目的: 研究Id 基因在肺癌和永生化支气管上皮细胞中的表达, 探讨其在肺癌细胞中表达的意义。方法: 利用半定量
RT- PCR 和Western blot 方法检测多种肺癌和永生化支气管上皮细胞中Id1- Id4mRNA 和Id1- Id4 蛋白的表达。结果: A549、NCI
- H460、NCI- H446、SK- MES- 1、Anip973 中Id1- Id3mRNA 均高表达, Id1 相对表达较强; 而AGZY 和MP- 184 中未表达Id1-
Id3mRNA; 腺癌细胞均表达了Id4mRNA, 而NCI- H446、SK- MES- 1 未表达Id4mRNA。A549, NCI- H460, NCI- H446, SK- MES-
1,Anip 973 中Id1, Id2, Id3 蛋白均高表达, A549, NCI- H446, Anip 973 中Id2 的表达高于NCI- H460, SK- MES- 1; A549, NCIH460,
Anip 973 有Id4 的高表达,NCI- H446, SK- MES- 1 无Id4 的表达, Id1- Id4 在AGZY 和MP- 184 中均未表达。结论: 4 种Id
基因均作为癌基因在肺癌的发生发展中发挥作用, Id1, Id2, Id3 与肺癌细胞的恶性程度以及增殖和转移密切相关, Id4 可做为肺腺
癌的检测标志物。 |
| 英文摘要: |
| Objective: To investigate the expression and significance of Id gene in lung cancer and immortalization bronchial epithelial
cells. Methods: The expressions of Id1- Id4 mRNA and their proteins were examined by semiquantitative RT- PCR and Western blot assay.
Results: Id1 to Id3 mRNA were high expressed in A549, NCI- H460, NCI- H446, SK- MES- 1and Anip973 cells but not in AGZY andMP
- 184 cells. The expression of Id1 was much higher than others. Id4 mRNA was expressed in adenocarcinoma cells but not in NCI- H446 and
SK- MES- 1 cells. The proteins of Id1 to Id3 were high expressed in A549, NCI- H460, NCI- H446, SK- MES- 1 and Anip973 cells. The
expression of Id2 in A549, NCI- H446 and Anip973 was higher than that in NCI- H460 and SK- MES- 1. Id4 was high expressed in A549,
NCI- H460 and Anip973 cells but not in NCI- H446 and SK- MES- 1 cells. There were no expressions from Id1 to Id4 in AGZY and MP-
184 cells. Conclusion: The four kinds of Id gene took a part in the progression and metastasis of lung cancer as an oncogene. Id1, Id2 and Id3
were closely related to the malignant degree, proliferation and metastasis of lung cancer . Id4 may be a mark of lung adenocarcinoma. |
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