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| 利用Fusarium poae制备T-2毒素的培养条件和提取方法 |
| Cultivation Condition and Extraction Method to Prepare T-2 Toxin Using Fusarium poae |
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| DOI： |
| 中文关键词: T-2毒素 镰孢茵 产毒培养 |
| 英文关键词: T-2 toxin Fusarium sp. toxin cultivation |
| 基金项目:国家自然科学基金，湛江市财政科技专项竞争性项目 |
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| 摘要点击次数: 72 |
| 全文下载次数: 16 |
| 中文摘要: |
| 比较2种不同优化方法对分离纯化梨孢镰孢菌（Fusarium poae）产生的T-2毒素的效果，并得到高纯度的T．2毒素，解决国内T-2毒素产业化问题。在优化Fusariumpoae产毒培养条件的基础上，对Burmeister的提取方法和Gregory培养基进一步优化以最大限度地提高T-2毒素的产率，从而得到高纯度并且高产率的T-2毒素。目标菌株最适产毒培养基成分：NH4H2PO4 1g，KCl 0．2g，MgSO4·7H2O 0．2g，葡萄糖10g，酵母膏5g，CuSO4·5H2O 0．005g，ZnSO4·7H2O 0．01g，H2O 1000mL。最佳产毒培养条件为8—25℃间隔12h变温、前期光照后期黑暗、前期振荡后期静止的培养条件下培养28d。在这种条件下可提高T-2毒素的产率达5倍。用丙酮氯仿进行分离，免疫亲和拄进行纯化得到高纯的T-2毒素。优化后的Burmeister提取方法提高了T-2毒素的产率。 |
| 英文摘要: |
| The effects to isolate and purify T-2 toxin produced by Fusarium poae(FP) with two different optimized methods were compared and obtained high purity of T-2 toxin to solve its industrialization problem in China.Based on the optimization of toxin producing conditions by FP,Burmeister extraction and Gregory medium were further optimized in order to improve the productivity of T-2 toxin to the maximum thereby to obtain high purity and high productivity of T-2 toxin.The results showed that the most suitable tox... |
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